Fluorescent Nano-Probes to Image Plant Cell Walls by Super-Resolution STED Microscopy.
Identifieur interne : 000E97 ( Main/Exploration ); précédent : 000E96; suivant : 000E98Fluorescent Nano-Probes to Image Plant Cell Walls by Super-Resolution STED Microscopy.
Auteurs : Gabriel Paës [France] ; Anouck Habrant [France] ; Christine Terryn [France]Source :
- Plants (Basel, Switzerland) [ 2223-7747 ] ; 2018.
Abstract
Lignocellulosic biomass is a complex network of polymers making up the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals, and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that requires some pretreatments and several types of catalysts to be transformed efficiently. Gaining more knowledge in the architecture of plant cell walls is therefore important to understand and optimize transformation processes. For the first time, super-resolution imaging of poplar wood samples has been performed using the Stimulated Emission Depletion (STED) technique. In comparison to standard confocal images, STED reveals new details in cell wall structure, allowing the identification of secondary walls and middle lamella with fine details, while keeping open the possibility to perform topochemistry by the use of relevant fluorescent nano-probes. In particular, the deconvolution of STED images increases the signal-to-noise ratio so that images become very well defined. The obtained results show that the STED super-resolution technique can be easily implemented by using cheap commercial fluorescent rhodamine-PEG nano-probes which outline the architecture of plant cell walls due to their interaction with lignin. Moreover, the sample preparation only requires easily-prepared plant sections of a few tens of micrometers, in addition to an easily-implemented post-treatment of images. Overall, the STED super-resolution technique in combination with a variety of nano-probes can provide a new vision of plant cell wall imaging by filling in the gap between classical photon microscopy and electron microscopy.
DOI: 10.3390/plants7010011
PubMed: 29415498
PubMed Central: PMC5874600
Affiliations:
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type="city">Reims</settlement></placeName><orgName type="university">Université de Reims Champagne-Ardenne</orgName></affiliation></author><author><name sortKey="Habrant, Anouck" sort="Habrant, Anouck" uniqKey="Habrant A" first="Anouck" last="Habrant">Anouck Habrant</name><affiliation wicri:level="4"><nlm:affiliation>FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, 2 Esplanade Roland Garros, 51100 Reims, France. anouck.habrant@inra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, 2 Esplanade Roland Garros, 51100 Reims</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Champagne-Ardenne</region><settlement type="city">Reims</settlement></placeName><orgName type="university">Université de Reims Champagne-Ardenne</orgName></affiliation></author><author><name sortKey="Terryn, Christine" sort="Terryn, Christine" uniqKey="Terryn C" first="Christine" last="Terryn">Christine Terryn</name><affiliation wicri:level="4"><nlm:affiliation>Plateforme d'Imagerie Cellulaire et Tissulaire (PICT), Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51100 Reims, France. christine.terryn@univ-reims.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Plateforme d'Imagerie Cellulaire et Tissulaire (PICT), Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51100 Reims</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Champagne-Ardenne</region><settlement type="city">Reims</settlement></placeName><orgName type="university">Université de Reims Champagne-Ardenne</orgName></affiliation></author></analytic><series><title level="j">Plants (Basel, Switzerland)</title><idno type="ISSN">2223-7747</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lignocellulosic biomass is a complex network of polymers making up the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals, and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that requires some pretreatments and several types of catalysts to be transformed efficiently. Gaining more knowledge in the architecture of plant cell walls is therefore important to understand and optimize transformation processes. For the first time, super-resolution imaging of poplar wood samples has been performed using the Stimulated Emission Depletion (STED) technique. In comparison to standard confocal images, STED reveals new details in cell wall structure, allowing the identification of secondary walls and middle lamella with fine details, while keeping open the possibility to perform topochemistry by the use of relevant fluorescent nano-probes. In particular, the deconvolution of STED images increases the signal-to-noise ratio so that images become very well defined. The obtained results show that the STED super-resolution technique can be easily implemented by using cheap commercial fluorescent rhodamine-PEG nano-probes which outline the architecture of plant cell walls due to their interaction with lignin. Moreover, the sample preparation only requires easily-prepared plant sections of a few tens of micrometers, in addition to an easily-implemented post-treatment of images. Overall, the STED super-resolution technique in combination with a variety of nano-probes can provide a new vision of plant cell wall imaging by filling in the gap between classical photon microscopy and electron microscopy.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29415498</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2223-7747</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>1</Issue><PubDate><Year>2018</Year><Month>Feb</Month><Day>06</Day></PubDate></JournalIssue><Title>Plants (Basel, Switzerland)</Title><ISOAbbreviation>Plants (Basel)</ISOAbbreviation></Journal><ArticleTitle>Fluorescent Nano-Probes to Image Plant Cell Walls by Super-Resolution STED Microscopy.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E11</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/plants7010011</ELocationID><Abstract><AbstractText>Lignocellulosic biomass is a complex network of polymers making up the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals, and materials. Because of its complex architecture, lignocellulose is a recalcitrant material that requires some pretreatments and several types of catalysts to be transformed efficiently. Gaining more knowledge in the architecture of plant cell walls is therefore important to understand and optimize transformation processes. For the first time, super-resolution imaging of poplar wood samples has been performed using the Stimulated Emission Depletion (STED) technique. In comparison to standard confocal images, STED reveals new details in cell wall structure, allowing the identification of secondary walls and middle lamella with fine details, while keeping open the possibility to perform topochemistry by the use of relevant fluorescent nano-probes. In particular, the deconvolution of STED images increases the signal-to-noise ratio so that images become very well defined. The obtained results show that the STED super-resolution technique can be easily implemented by using cheap commercial fluorescent rhodamine-PEG nano-probes which outline the architecture of plant cell walls due to their interaction with lignin. Moreover, the sample preparation only requires easily-prepared plant sections of a few tens of micrometers, in addition to an easily-implemented post-treatment of images. Overall, the STED super-resolution technique in combination with a variety of nano-probes can provide a new vision of plant cell wall imaging by filling in the gap between classical photon microscopy and electron microscopy.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Paës</LastName><ForeName>Gabriel</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, 2 Esplanade Roland Garros, 51100 Reims, France. gabriel.paes@inra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Habrant</LastName><ForeName>Anouck</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, 2 Esplanade Roland Garros, 51100 Reims, France. anouck.habrant@inra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Terryn</LastName><ForeName>Christine</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Plateforme d'Imagerie Cellulaire et Tissulaire (PICT), Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51100 Reims, France. christine.terryn@univ-reims.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>02</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Plants (Basel)</MedlineTA><NlmUniqueID>101596181</NlmUniqueID><ISSNLinking>2223-7747</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">STED</Keyword><Keyword MajorTopicYN="N">fluorescent probe</Keyword><Keyword MajorTopicYN="N">lignocellulose</Keyword><Keyword MajorTopicYN="N">plant cell wall</Keyword><Keyword MajorTopicYN="N">poplar</Keyword><Keyword MajorTopicYN="N">super-resolution microscopy</Keyword></KeywordList><CoiStatement>The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>01</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>01</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>02</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId 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